CRISPR/Cas9 guide RNA generator

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Find protospacer adjacent motifs (PAM, 5′-NGG-3′) and generate Cas9 guide RNAs, either in a sequence you paste or in a region of one of 25 bacterial genomes.

Where to search

Source

Strand

Genome region (bp) to

Name of your sequence (optional)

DNA sequence (5′ to 3′), when searching your own sequence

Load a .txt file

If you use this tool, please cite: Kaloudas D., Pavlova N., Penchovsky R. EBWS: Essential Bioinformatics Web Services for Sequence Analyses. IEEE/ACM Transactions on Computational Biology and Bioinformatics (2019). Created together with Dr. Dimitrios Kaloudas (dimitris.kaloudas@gmail.com).

GCRISPR/Cas9 guide RNA generator

CRISPR/Cas9 guide RNA generator